立命館大学 石水毅研究室

研究業績

発表論文

  1. Matsumoto, N., Takenaka, Y., Wachananawat, B., Kajiura, H., Imai, T., and Ishimizu, T. Rhamnogalacturonan I galactosyltransferase: Detection of enzyme activity and its hyperactivation. Plant Physiol. Biochem. 142, 173-178 (2019) doi: 10.1016/j.plaphy.2019.07.008
  2. Fujimori, T., Matsuda, R., Suzuki, M., Takenaka, Y., Kajiura, H., Takeda, Y., Ishimizu, T. Practical preparation of UDP-apiose and its application for studying apiosyltransferase. Carbohydr. Res. 477, 20-25 (2019) doi: 10.1016/j.carres.2019.03.011
  3. 竹中悠人、梶浦裕之、石水毅 植物細胞壁多糖の生合成ー新規ペクチン生合成糖転移酵素の発見と今後の展望 化学と生物 57(2), 88-94 (2019)
  4. 石水毅 ペクチン合成のしくみ解明が植物の陸上進出を知る鍵に! 化学 73(10), 73 (2018)
  5. 竹中悠人、石水毅 植物の細胞壁の成分であるペクチンの生合成にかかわるラムノース転移酵素の同定および陸上植物に特異的な新規の糖転移酵素ファミリーの発見 ライフサイエンス新着論文レビュー (2018) doi: 10.7875/first.author.2018.080
  6. Takenaka, Y., Kato, K., Ogawa-Ohnishi M., Tsuruhama, K., Kajiura, H., Yagyu, K., Takeda, A., Takeda, Y., Kunieda, T., Hara-Nishimura, I., Kuroha, T., Nishitani, K., Matsubayashi, Y., and Ishimizu, T. Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family. Nature Plants 4, 669-676 (2018) doi: 10.1038/s41477-018-0217-7
  7. Rahman, M.Z., Tsujimori, Y., Maeda, M., Hossain, M.A., Ishimizu, T., Kimura, Y. Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase SI-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans. J. Biochem. 164, 53-63 (2018) doi: 10.1093/jb/mvy029
  8. Kato, S., Hayashi, M., Kitagawa, M., Kajiura, H., Maeda, M., Kimura, Y., Igarashi, K., Kasahara, M. and Ishimizu, T. Degradation pathway of plant complex-type N-glycans: Identification and characterization of a key α1,3-fucosidase from Glycoside Hydrolase family 29. Biochem. J. 475. 305-317 (2018) doi:
  9. Ohashi, T., Jinno, J., Inoue, Y., Ito, S., Fujiyama, K., Ishimizu, T. A polygalacturonase localized in the Golgi apparatus in Pisum sativum. J. Biochem. 162, 193-201 (2017) doi: 10.1093/jb/mvx014
  10. Rahman, M.Z., Maeda, M., Itano, S., Hossain, M.A., Ishimizu, T., Kimura, Y. Molecular characterization of tomato α1,3/4-fucosidase, a member of glycosyl hydrolase family 29, involved in degradation of plant complex type N-glycans. J. Biochem. 161, 421-432 (2017) doi: 10.1093/jb/mvw089
  11. Uehara, Y., Tamura, S., Maki, Y., Yagyu, K., Mizoguchi, T., Tamiaki, H., Imai, T., Ishii, T., Ohashi, T., Fujiyama, K., Ishimizu, T. Biochemical characterization of rhamnosyltransferase involved in biosynthesis of pectic rhamnogalacturonan I in plant cell wall. Biochem. Biophys. Res. Commun. 486, 130-136 (2017) doi: 10.1016/j.bbrc.2017.03.012
  12. 石水毅 (分担執筆)植物細胞壁実験法(石井忠、石水毅、梅澤俊明、加藤陽治、岸本崇生、小西照子、松永俊朗 編著) 弘前大学出版会 (2016)
  13. Okamoto, R., Kimura, M., Ishimizu, T., Izumi, M., Kajihara, Y. Semisynthesis of a post-translationally modified protein by using chemical cleavage and activation of an expressed fusion polypeptide. Chem. Eur. J. 20, 10425-10430 (2014) doi: 10.1002/chem.201403035
  14. 長谷川慎、前田衣織、有馬一成、石水 毅 生体高分子の基礎~はじめてのバイオ分子化学~ 実教出版 (2014)
  15. Ishimizu, T. Plant N-glycans and their degrading enzymes. In Glycoscience: Biology and Medicine (Eds. Taniguchi N., Endo, T., Hart, G., Seeberger, P. and Wong, C.-H.) Springer, Heidelberg, Germany, pp.1111-1117 (2014) doi: 10.1007/978-4-431-54841-6_69
  16. 石水毅 植物N型糖鎖の分解とその生理機能 バイオサイエンスとインダストリー 71, 220-223 (2013)
  17. 石水毅 N型糖鎖に作用する植物マンノシダーゼ 応用糖質学会誌 3, 93-98 (2013) doi: 10.5458/bag.3.1_93
  18. 石水毅 (分担執筆)植物細胞壁(西谷和彦、梅澤俊明編) 講談社 (2013)
  19. Ishimizu, T. The N-glycan degradation pathway in plants. Glycoscience 2008-2012 pp.68-69. (2012)
  20. Kimura, M., Izumi, M., Okamoto, R., Ishimizu, T., and Kajihara, Y. New strategy for the synthesis of glycoprotein: A single expression method of two peptide segments encoded as a tandem sequence in plasmid and its conversion to peptide-αthioester. Peptide Science 48, 15-16 (2012)
  21. 石水 毅 植物N-結合型糖鎖の分解機構 生化学 83, 1087-1099 (2011)
  22. Arakawa, J., Uegaki, M., and Ishimizu, T. Effects of L-arginine on solubilization and purification of plant membrane proteins. Protein Expr. Purif. 80, 91-96. (2011)
  23. 石水 毅 八方美人なアルギニンーアルギニンがタンパク質凝集抑制に効くワケ 化学 66(10), 60-61 (2011)
  24. 石水 毅 路傍の花 日本生化学会「若手研究者に聞く」(2011)
  25. Ishimizu, T., Kodama, H., Ando, T., and Watanabe, M. Molecular evidence that most RNAs required for germination and pollen tube growth are stored in the mature pollen grain in petunia. Genes Genet. Syst. 85, 259-263 (2010)
  26. Fujita, M., Horiuchi, Y., Ueda, Y., Mizuta, Y., Kubo, T., Yano, K., Yamaki, S., Tsuda, K., Nagata, T., Niihama, M., Kato, H., Kikuchi, S., Hamada, K., Mochizuki, T., Ishimizu, T., Iwai, H., Tsutsumi, N., and *Kurata, N. Rice reproductive expression atlas: The deepest transcriptome analysis in reproductive development of rice. Plant Cell Physiol. 51, 2060-2081 (2010)
  27. *Yoneda, A., Ito, T., Higaki, T., Kutsuna, N., Saito, T., Ishimizu, T., Osada, H., Hasezawa, S., Matsui, M., and Demura, T. Cobtorin target analysis reveals that pectin functions in deposition of cellulose microfibrils parallel to cortical microtubules in a manner dependent on the methylesterification ratio of pectin and its distribution. Plant J. 64, 657-667 (2010)
  28. Uegaki, M., Yasui, K., and *Ishimizu, T. Effective solubilization of polygalacturonic acid synthase by ribonuclease treatment of cell homogenate. Plant Biotechnol. 27, 289-291 (2010)
  29. Park, J.I., Ishimizu, T., Suwabe, K., Sudo, K., Masuko, H., Hakozaki, H., Nou, I.S., Suzuki, G., and *Watanabe, M. UDP-glucose pyrophosphorylase is rate limiting in vegetative and reproductive phases in Arabidopsis thaliana. Plant Cell Physiol. 51, 981-996 (2010) (First three authors equally contributed to this work.)
  30. Yasui, K., Uegaki, M., Shiraki, K., and *Ishimizu, T. Enhanced solubilization of membrane proteins by alkylamines and polyamines. Protein Sci. 19, 486-493 (2010)
  31. Yasui, K., Jinno, J, Ohashi, T., and *Ishimizu, T. In vitro synthesis of polygalacturonic acid. In Pectins and Pectinases (Eds. Voragen F., Schols, H, and Visser, R.) Kluwer, Dordrecht, the Netherlands, pp. 167-175 (2009)
  32. *長谷純宏、長束俊治、中北愼一、石水 毅 ピリジルアミノ化による糖鎖解析〜糖鎖多様性の解析に向けて〜 大阪大学出版会 (2009)
  33. *Ishimizu, T. Progress towards understanding xylan biosynthesis. Trends Glycosci. Glycotech. 20, 171-172 (2008)
  34. Hobo, T., Suwabe, K., Aya, K., Suzuki, G., Yano, K., Ishimizu, T., Fujita, M., Kikuchi, S., Hamada, K., Miyano, M., Fujioka, T., Kaneko, F., Kazama, T., Mizuta, Y., Takahashi, H., Shiono, K., Nakazono, M., Tsutsumi, N., Nagamura, Y., Kurata, N., *Watanabe, M., and *Matsuoka, M. Various spatiotemporal expression profiles of anther-expressed genes in rice. Plant Cell Physiol. 49, 1417-1428 (2008) (表紙写真掲載)
  35. Norioka, S., Oneyama, C., Takuma, S., Shinkawa, T., *Ishimizu, T., Nakanishi, T., Sakiyama, F. Purification and characterization of a non-S-RNase and S-RNases from styles of Japanese pear (Pyrus pyrifolia). Plant Physiol. Biochem. 45, 878-886 (2007)
  36. *Ishimizu, T., Hashimoto, C., Takeda, R., Fujii, K., and Hase, S. A novel α1,2-L-fucosidase acting on xyloglucan oligosaccharides is associated with endo-β-mannosidase. J. Biochem. 142, 721-729 (2007) (日本生化学会JB論文賞受賞)
  37. Shimamura, K., Ishimizu, T., Nishimura, K., Matsubara, K., *Kodama, H., Watanabe, H., Hase, S., and Ando, T. Analysis of expressed sequence tags from petunia flowers. Plant Sci. 173, 495-500 (2007)
  38. Ohashi, T., *Ishimizu, T., Akita, K., and Hase S. Stabilization and minimum active component of polygalacturonic acid synthase involved in pectin biosynthesis. Biosci. Biotechnol. Biochem. 71, 2291-2299 (2007)
  39. Ishimizu, T., Sano, K., Uchida, T., Teshima, H., Omichi, K., Hojo, H., Nakahara, Y., and *Hase, S. Purification and substrate specificity of UDP-D-xylose: β-D-glucoside α-1,3-D-xylosyltransferase involved in the biosynthesis of the Xylα1-3Xylα1-3Glc1β-O-Ser on epidermal growth factor-like domains. J. Biochem. 141, 593-600 (2007)
  40. *Ishimizu, T. The discovery of components of the plant cell wall biosynthesis systems. Trends Glycosci. Glycotech. 18, 401-402 (2006)
  41. *Ishimizu, T. and Hase, S. Synthesis of β-mannosides using endo-β-mannosidase. In Endoglycosidases (Eds. Endo, M., Yamamoto, K., Takagaki, K., and Hase, S.) Springer, Berlin, Germany, pp. 162-171 (2006)
  42. *Ishimizu, T. and Hase, S. Endo-β-mannosidase. In Endoglycosidases (Eds. Endo, M., Yamamoto, K., Takagaki, K., and Hase, S.) Springer, Berlin, Germany, pp.74-83 (2006)
  43. *Ishimizu, T. and Hase, S. Endo-β-mannosidase, a plant enzyme acting on N-glycans. Trends Glycosci. Glycotech. 18, 65-73 (2006)
  44. Ishimizu, T., Hashimoto, C., Kajihara, R., and *Hase, S. A retaining endo-β-mannosidase from a dicot plant, cabbage. J. Biochem. 139, 1035-1043. (2006)
  45. Ohashi, T. Cramer, N., *Ishimizu, T., and Hase, S. Preparation of UDP-galacturonic acid using UDP-sugar pyrophosphorylase. Anal. Biochem. 352, 182-187 (2006)
  46. *Ishimizu, T. and Hase, S. Substrate recognition by sugar chain-related enzymes: recognition of a large area of substrates and its strictness and tolerance. Trends Glycosci. Glycotech. 17, 215-227 (2005)
  47. Sasaki, A., Ishimizu, T., Geyer, R., and *Hase, S. Synthesis of -mannosides using the transglycosylation activity of endo-β-mannosidase from Lilium longiflorum. FEBS J. 272, 1660-1668 (2005)
  48. Ishimizu, T., Uchida, T., Sano, K., and *Hase, S. Chemical synthesis of uridine 5'-diphospho-α-D-xylopyranose. Tetrahedron Asymm. 16, 309-311 (2005)
  49. Sasaki, A., Ishimizu, T., and *Hase, S. Substrate specificity and molecular cloning of the lily endo-β-mannosidase acting on N-glycan. J. Biochem. 137, 87-93 (2005)
  50. *Ishimizu, T. and Hase, S. Pectin biosynthesis. In Advances in Plant Physiology Vol. 7 (Ed. Hemantaranjan, A.), Scientific Pubishers. Jodhpur, India, pp. 175-193 (2004)
  51. Kitamura, M., *Hojo, H., Nakahara, Y., Ishimizu, T., and Hase, S. Synthesis and conformational characterization of the epidermal growth factor-like domain of blood coagulation factor IX carrying xylosyl-glucose. Glycoconj. J. 21, 197-203 (2004)
  52. Ishimizu, T., Sasaki, A., Okutani, S., Maeda, M., Yamagishi, M. and *Hase, S. Endo-β-mannosidase, a plant enzyme acting on N-glycan: purification, molecular cloning, and characterization. J. Biol. Chem. 279, 38555-38562 (2004) (表紙写真掲載)
  53. Takemura, T., *Hojo, H., Nakahara, Y., Ishimizu, T., and Hase, S. Application of Fmoc-amino acid carrying an unmasked carbohydrate to the synthesis of the epidermal growth factor-like domain of bovine blood coagulation factor IX. Org. Biomol. Chem. 2, 133-136 (2004)
  54. Castillo, C., *Takasaki, T., Saito, T., Ishimizu, T., Norioka, S., and Nakanishi, T. S-RNase based PCR-RFLP system for S-genotype identification in Japanese pear. Acta Horticulturae 587, 449-458 (2002)
  55. Akita, K., Ishimizu, T., Tsukamoto, T., Ando, T., and *Hase, S. Successive glycosyltransfer activity and enzymatic characterization of pectic polygalacturonate 4-α-galacturonosyltransferase solubilized from pollen tubes of Petunia axillaris using pyridylaminated oligogalacturonates as substrates. Plant Physiol. 130, 374-379 (2002)
  56. Yamagishi, M., Ishimizu, T., Natsuka, S., and *Hase, S. Co(II)-regulated substrate specificity of cytosolic α-mannosidase. J. Biochem. 132, 253-256 (2002)
  57. Kiyozumi, D., Ishimizu, T., Nakanishi, T., and *Norioka, S. Pollen UDP-glucose pyrophosphorylase showing polymorphism well-correlated to the S genotype of Pyrus pyrifolia. Sex. Plant Reprod. 14, 315-323 (2002)
  58. Skirpan, A.L., McCubbin, A.G., Ishimizu, T., Wang, X., Hu, Y., Dowd, P.E., Ma, H. and *Kao, T-h. Isolation and characterization of kinase interacting protein 1, a pollen protein that interacts with the kinase domain of PRK1, a receptor-like kinase of petunia. Plant Physiol. 126, 1480-1492 (2001)
  59. Norioka, N., Katayama, H., Matsuki, T., Ishimizu, T., Takasaki, T., Nakanishi, T., *Norioka, S. Sequence comparison of the 5' flankling regions of Japanese pear (Pyrus pyrifolia) S-RNases associated with gametophytic self-incompatibility. Sex. Plant Reprod. 13 , 289-291 (2001)
  60. Ishimizu, T., Mitsukami, Y., Shinkawa, S., Natsuka, S., Hase, S., Miyagi, M., Sakiyama, F., and *Norioka, S. Presence of asparagine-linked N-acetylglucosamine and chitobiose in Pyrus pyrifolia S-RNases associated with gametophytic self-incompatibility. Eur. J. Biochem. 263, 624-634 (1999)
  61. Ishimizu, T., Inoue, K., Shimonaka, M., Saito, T., Terai, O., and *Norioka S. PCR-based method for determination of S-genotype of Japanese pear cultivar. Theor. Appl. Genet. 98, 961-967. (1999)
  62. Kiyozumi, D., Ishimizu, T., Nakanishi, T., Sakiyama, F., and *Norioka, S. Molecular Cloning and nucleotide sequencing of a cDNA encoding UDP-glucose pyrophosphorylase of Japanese pear (Pyrus pyrifolia) (Accession No. AB013353) (PGR99-006). Plant Physiol. 119, 364 (1999)
  63. *乗岡茂巳、石水 毅、崎山文夫 ニホンナシ自家不和合性とリボヌクレアーゼ 蛋白質工学基礎研究センターだより 19, 22-28 (1998)
  64. Ishimizu, T., Endo, T., Yamaguchi-Kabata, Y., Nakamura, K.T., Sakiyama, F., and *Norioka, S. Identification of regions in which positive selection may operate in S-RNase of Rosaceae: Implication for S-allele-specific recognition sites in S-RNase. FEBS Lett. 440, 337-342 (1998)
  65. Ishimizu, T., Shinkawa, T., Sakiyama, F., and *Norioka, S. Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility. Plant Mol. Biol. 37, 931-941 (1998)
  66. Ishimizu, T., Norioka, S., Nakanishi, T., and *Sakiyama, F. S-genotype of Japanese pear 'Hosui'. J. Japan. Soc. Hort. Sci. 67, 35-38 (1998)
  67. Ishimizu, T., Norioka, S., Kanai, M., Clarke, A.E., and Sakiyama, F. Location of cysteine and cystine residues in tobacco S6-RNase and Japanese pear S4-RNase associated with gametophytic self-incompatibility. Eur. J. Biochem.242, 627-635 (1996) doi: 10.1111/j.1432-1033.1996.0627r.x
  68. Norioka, N., Norioka, S., Ohnishi, Y., Ishimizu, T., Oneyama, C., Nakanishi, T., and Sakiyama, F. Molecular cloning and nucleotide sequences of cDNAs encoding S-alleles specific pistil RNases in a self-incompatible cultivar and its self-compatible mutant of Japanese pear (Pyrus pyrifolia Nakai). J. Biochem.120, 335-345 (1996) (日本生化学会JB論文賞受賞) doi: 10.1093/oxfordjournals.jbchem.a021418
  69. Ishimizu, T., Sato, Y., Saito, T., Yoshimura, Y., Norioka, S., Nakanishi, T., and Sakiyama, F. Identification and partial amino acid sequences of seven S-RNases associated with self-incompatibility of Japanese pear, Pyrus pyrifolia Nakai. J. Biochem.120, 326-334 (1996) doi: 10.1093/oxfordjournals.jbchem.a021417
  70. Ishimizu, T., Miyagi, M., Norioka, S., Liu, Y.-H., Clarke, A.E., and Sakiyama, F. Identification of histidine 31 and cysteine 95 in the active site of self-incompatibility associated S6-RNase in Nicotiana alata. J. Biochem.118, 1007-1013 (1995) doi: 10.1093/jb/118.5.1007
  71. Norioka, N., Ohnishi, Y., Norioka, S., Ishimizu, T., Nakanishi, T., and Sakiyama, F. Nucleotide sequences of cDNAs encoding S2- and S4-RNases (D49257 and D49528 for EMBL) from Japanese pear (Pyrus Pyriforia Nakai). Plant Physiol.108, 1343 (1995) doi:

受賞歴

  1. 弘前大学出版会賞 (2017)
  2. 大阪大学功績賞 (2011)
  3. 日本生化学会 奨励賞 (2010)
  4. 日本生化学会 Journal of Biochemistry論文賞 (2008)
  5. 日本生化学会 柿内三郎記念研究奨励賞 (2005)
  6. 日本糖質学会 ポスター賞 (2005)
  7. 井上科学振興財団 井上研究奨励賞 (2001)
  8. 日本生化学会 Journal of Biochemistry 論文賞 (1997)

学生の受賞

学外表彰

  1. 松廣拓真 第10回植物細胞壁研究者ネットワーク研究会優秀ポスター発表賞 2016年10月4日
  2. 髙田和拓 第10回植物細胞壁研究者ネットワーク研究会優秀ポスター発表賞 2016年10月4日
  3. 加藤耕平 第10回植物細胞壁研究者ネットワーク研究会最優秀口頭発表賞 2016年10月4日
  4. 加藤大詞 第9回植物細胞壁研究者ネットワーク研究会優秀ポスター発表賞 2015年9月15日
  5. 髙田和拓 第9回植物細胞壁研究者ネットワーク研究会優秀口頭発表賞 2015年9月15日
  6. 加藤俊 第87回日本生化学会大会若手優秀発表賞 2014年10月16日
  7. 上原洋平 第8回植物細胞壁研究者ネットワーク研究会最優秀研究発表賞 2014年9月26日
  8. 上原洋平 サントリー生有研シンポジウムBest Discussion賞 2013年7月17日

学内表彰

  1. 加藤耕平 生命科学研究科修士論文優秀賞 2018年3月22日
  2. 平岡晋 父母教育後援会学部表彰 2017年3月22日
  3. 加藤俊 生命科学研究科修士論文優秀賞 2016年3月22日
  4. 松廣拓真 父母教育後援会学部表彰 2016年3月22日
  5. 加藤耕平 西園寺育英奨学生 2015年7月8日
  6. 髙田和拓 父母教育後援会学部表彰 2015年3月21日
  7. 加藤俊 父母教育後援会学部表彰 2014年3月22日
  8. 鶴浜和奈 西園寺育英奨学生 2013年7月10日